Journal: BMC Immunology

Article Title: Natural killer cells are crucial for the efficacy of Icon (factor VII/human IgG1 Fc) immunotherapy in human tongue cancer

doi: 10.1186/1471-2172-11-49

Figure Lengend Snippet: Lysis of TCA8113 cancer cells by Icon-dependent NK cell-mediated ADCC and complement-mediated CDC in vitro . a . Isolation of murine NK cells from a single cell suspension of splenocytes from SCID/CB-17 mice. b . Icon-dependent NK cell-mediated ADCC. c . Icon-dependent complement-mediated cytotoxicity. Note that % specific lysis was derived from the % of cytotoxicity of Icon + NK cell or Icon + complement subtracted by % of cytotoxicity of NK cell alone or complement alone. P values and no significance (ns) were analysed by unpaired t test (b) or one way ANOVA with Tukey's Multiple Comparison Test (c). Data are representative of two experiments.

Article Snippet: 1:4 diluted rabbit complement MA (Cedarlane Laboratories) in HBSS was added to each well that had been incubated with mouse Icon protein (Icon-CDC experimental wells) or HBSS buffer (complement alone wells) and incubated at 37°C for 60 min. For the ADCC assay, freshly isolated mouse NK and non-NK cells were added to the plates and incubated at 37°C for 4 hrs.

Techniques: Lysis, In Vitro, Isolation, Suspension, Flow Cytometry Complement-Mediated Cytotoxicity Assay, Derivative Assay, Comparison

Journal: BMC Immunology

Article Title: Natural killer cells are crucial for the efficacy of Icon (factor VII/human IgG1 Fc) immunotherapy in human tongue cancer

doi: 10.1186/1471-2172-11-49

Figure Lengend Snippet: Icon protein has no effect on cancer cell proliferation in the absence of NK cell and complement . After incubation with mouse Icon protein for 1 hr followed by overnight growth, cancer cell proliferation was determined by CellTiter One Solution reagent, as described in Methods. P values obtained were of no significance as determined by one-way ANOVA with Tukey's Multiple Comparison Test. Data are representative of two experiments.

Article Snippet: 1:4 diluted rabbit complement MA (Cedarlane Laboratories) in HBSS was added to each well that had been incubated with mouse Icon protein (Icon-CDC experimental wells) or HBSS buffer (complement alone wells) and incubated at 37°C for 60 min. For the ADCC assay, freshly isolated mouse NK and non-NK cells were added to the plates and incubated at 37°C for 4 hrs.

Techniques: Incubation, Comparison

Journal: Scientific reports

Article Title: A nonadjuvanted HLA-restricted peptide vaccine induced both T and B cell immunity against SARS-CoV-2 spike protein.

doi: 10.1038/s41598-024-71663-1

Figure Lengend Snippet: Fig. 4. In vivo immune responses of injected UC peptides and complement activity of immunized serum. (a) BALB/c mice were intramuscularly administered 3 doses of 50 µg of UC-100 (n = 3) or 400 µg of UC-152 (n = 4) 14 days apart. (b–d) The serum of UC-100 mice was collected pre-(Day-1) and posttreatment (Day41) and tested in duplicate and represented as the mean for the levels of (b) total IgG; (c) specific IgG against UC-100 peptide; (d) specific IgG against SARS-CoV-2 spike trimer protein. (e–g) The serum of UC-152 mice was collected pre-(Day-1) and posttreatment (Day41) and tested in duplicate and represented as the mean for the levels of (e) total IgG; (f) specific IgG against UC-152 peptide; (g) specific IgG against SARS-CoV-2 spike trimer protein. (h,i) Antibody-mediated complement activity was tested in duplicate and is represented as the mean for pre- and postserum of mice vaccinated with UC-100 and UC-152 peptides, respectively. The complement activity was expressed as the IC50 of cytotoxicity of the (h) UC-100-vaccinated group and (i) UC-152-vaccinated group. Significant differences are indicated by asterisks (unpaired one-tailed Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: A final concentration of 10% young rabbit complement (CL3441-S50-R, Cedarlane, Ontario, Canada) was added to each well and incubated for an additional 30 min at 37 °C and 5% CO2.

Techniques: In Vivo, Injection, Activity Assay, One-tailed Test

Journal: Scientific reports

Article Title: A nonadjuvanted HLA-restricted peptide vaccine induced both T and B cell immunity against SARS-CoV-2 spike protein.

doi: 10.1038/s41598-024-71663-1

Figure Lengend Snippet: Fig. 5. In vivo immune responses of oral UC peptides and complement activity of immunized serum. (a) BALB/c mice (n = 5) were orally administered 3 doses of 200 µg UC-100 14 days apart. (b–d) The serum of UC-100 mice was collected pre-(Day-1) and posttreatment (Day 41) and tested in duplicate and represented as the mean for the levels of (b) total IgG; (c) specific IgG against UC-100 peptide; (d) specific IgG against SARS-CoV-2 spike trimer protein; (e) The antibody-mediated complement activity was tested in duplicate and represented as the mean for pre- and postserum of mice vaccinated with UC-100. The complement activity was expressed as the IC50 of cytotoxicity. (f,g) The serum of UC-100 mice was collected pre-(Day-1) and posttreatment (Day 41) and tested in duplicate and represented as the mean for the levels of (f) total IgA and (g) specific IgA against UC-100 peptide. Significant differences are indicated by asterisks (unpaired one-tailed Student’s t test; *p < 0.05, **p < 0.01, ***p < 0.001).

Article Snippet: A final concentration of 10% young rabbit complement (CL3441-S50-R, Cedarlane, Ontario, Canada) was added to each well and incubated for an additional 30 min at 37 °C and 5% CO2.

Techniques: In Vivo, Activity Assay, One-tailed Test

Journal: Scientific reports

Article Title: A nonadjuvanted HLA-restricted peptide vaccine induced both T and B cell immunity against SARS-CoV-2 spike protein.

doi: 10.1038/s41598-024-71663-1

Figure Lengend Snippet: Fig. 6. Summary of the SARS-CoV-2 HLA-restricted peptide T-cell vaccine. The possible mechanistic immune response to HLA-restricted peptides has been elucidated. The 9-mer UC peptides can be primed onto both HLA class I and II molecules, and the immune response is triggered for both humoral and cellular immunity. Through HLA class I molecule binding, CTLs can be activated, and an increase in IFN-γ cytokines and specific lytic activity have been observed. Through HLA class II molecule binding, B-cells are activated, and specific antibodies are produced in accordance with complement cytotoxicity activity.

Article Snippet: A final concentration of 10% young rabbit complement (CL3441-S50-R, Cedarlane, Ontario, Canada) was added to each well and incubated for an additional 30 min at 37 °C and 5% CO2.

Techniques: Binding Assay, Activity Assay, Produced